MOLECULAR IDENTIFICATION OF KAPPAPHYCUS SPECIES COLLECTED IN ILOILO AND ROMBLON
John Rey T. Rasgo1, Jonalyn P. Mateo1,2*, Rema C. Sibonga2,3, Victor Marco Emmanuel N. Feriols2, Anicia Q. Hurtado2, Maria Rovilla J. Luhan2
1Institute of Marine Fisheries and Oceanology, University of the Philippines Visayas Miagao, Philippines 5023
2Institute of Aquaculture, University of the Philippines Visayas Miagao, Philippines 5023
3Center for Chemical Biology and Biotechnology, University of San Agustin, Iloilo City, 5000, Philippines
*Corresponding author: jpmateo@up.edu.ph
For more than 50 years, Kappaphycus, specifically K. alvarezii and K. striatus, have been cultured in the Philippines. Both species showed different varieties and color morphotypes which led to confusion, difficulties in species identification and eventually resulted to misidentification owing to its morphologically diverse nature, lack of clear diagnostic on morphological characteristics and inconsistent use of local and commercial names. The use of molecular markers has been proven to be effective in delineating Kappaphycus species from each other. In this study, cultivated seaweeds from Iloilo and Romblon were collected, morphologically described and analyzed for molecular identifiction. Genomic DNA was extracted using a modified CTAB protocol and amplified by polymerase chain reaction (PCR) using COI-5P and cox 2-3 spacer as molecular markers. The PCR products were purified and sent to Philippine Genome Center Visayas for sequencing. Based on external morphology, specimen from Iloilo and Romblon were initially identified as K. alvarezii (tambalang variety) and K. striatus (kulot variety), respectively. The species-level affiliation of the generated sequences was initially determined using BLAST analysis and confirmed by phylogenetic analysis. Both Iloilo and Romblon specimens were K. alvaezii based on BLAST results. The maximum likelihood tree revealed that the two specimens are of the same clade with other K. alvarezii specimens. In summary, kulot and tambalang, though morphologically different are both K. alvarezii. Thus, molecular technique is an important tool in proper identification of the cultivated seaweed.
Keywords: Color morphotypes, Kappaphcyus, Phylogenetic analysis