PHARMACOKINETIC AND ELIMINATION OF FLORFENICOL, DOXYCYCLINE, AMOXICILLIN IN STRIPED CATFISH (Pangasianodon hypophthalmus)
Vinh Q. Pham1,*, Thinh Q. Nguyen1, Luan C. Nguyen1, Phu M. Tran1
1Can Tho University, 3/2 street, Xuan Khanh Ward, Ninh Kieu District, Can Tho City, Viet Nam
*Corresponding author: phamquangvinh@ymail.com
Study on pharmacokinetic and elimination of florfenicol (FF), doxycycline (DOX), amoxicillin (AMX) in striped catfish (90-100 g/fish) was done in order to determine the pharmacokinetic data and withdraw times of the antibiotic residue, to rationalize the use of antibiotic. The study included two experiments. In the first experiment, pharmacokinetic data of FF (10 mg/kg fish), DOX (20 mg/kg fish) và AMX (50 mg/kg fish) in plasma, liver and kidney were evaluated. Fish were orally administered antibiotic through pumping into fish stomach. Sampling of plasma, liver and kidney was done after 30 min, 1, 2, 4, 6, 12 and 24 h for FF; 30 min, 1, 2, 4, 6, 12, 24, 48 and 96 hours (h) for DOX; 30 min, 1, 2, 4, 8 and 18 h for AMX. Pharmacokinetic data analysis was done following Noncomparkmental PK analysis. Results showed that the maximum FF concentration in catfish plasma and time to reach the maximum concentration was lower than other fish species. PK of FF in fish plasma included Cmax=342 µg/L, Tmax=3.7 h and AUC0-inf=3.44 µg.h/kg. There was metabolism of florfenicol into florfenicol amine in fish plasma, liver and kidney. For DOX, Cmax=842 µg/L, Tmax=6.2 h, AUC0-inf=7.320 µg.h/kg. The PK data of AMX included Cmax=127µg/L, Tmax=1 h, AUC0-8 h=375 µg.h/L. The DOX and AMX concentrations in kidney were higher than liver, and with longer time of accumulation. In the second experiment, the elimination of FF, DOX and AMX in fish muscle was determined after 5 consecutive days feeding medicated feed with the similar dose as the first experiment. Fish muscle was collected before feeding medicated feed, day one and day 5 during feeding medicated feed and days 1, 3, 7, 14 and 30 after feeding medicated feed. Results showed that FF and FFA levels were 872 µg/kg and 106 µg/kg, respectively, after 5 days feeding medicated feed. After 24 h stop feeding medicated feed, total FF and FFA concentration in fish muscle was below maximum residue limit, assigned by the EU (1,000 µg/kg). For DOX, after 7 stop feeding medicated feed, DOX level in fish muscle was 25.0±4.88 µg/kg, below 100 µg/kg following EU regulation. Similar to FF, AMX eliminated very fast after 5 days feeding medicated feed. One day after stop feeding AMX, AMX residue was not detected in fish muscle (LOD=5 µg/kg).
Keywords: amoxicillin, doxycycline, florfenicol, Pangasianodon hypophthalmus, pharmacokinetic, striped catfish, residue